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71.
Gustav Kramer 《Journal of Ornithology》1932,80(3):329-342
Ohne Zusammenfassung 相似文献
72.
Dr. Gustav v. Pernhoffer 《Plant Systematics and Evolution》1896,46(5):196-197
Ohne Zusammenfassung 相似文献
73.
Karin Johannesen Joseph W. Depierre Anders Bergstrand Gustav Dallner Lars Ernster 《Biochimica et Biophysica Acta (BBA)/General Subjects》1977,496(1):115-135
Optimal conditions for the preparation of relatively pure microsomes and microsomal subfractions from rat lung have been determined. The most important of these conditions is homogenization of a 20% (w/v) suspension of lung tissue in 0.44 M sucrose/1% (w/v) bovine serum albumin with four up-and-down strokes at 440 rev./min in a Potter-Elvehjem homogenizer. The 10 000 × g supernatant prepared from this homogenate can be centrifuged at 105 000 × g to obtain total microsomes or subfractionated into rough and smooth microsomes on a Cs+-containing discontinuous sucrose gradient. The total, rough and smooth microsomes have been characterized in terms of their chemical composition, enzymatic activity, and morphology. These preparations should prove useful in studies of various enzymes in lung (e.g. benzpyrene monooxygenase, epoxide hydrase, enzymes of phospholipid and ascorbic acid synthesis) and in subfractionations designed to reveal heterogeneites in the lateral plane of the lung endoplasmic reticulum. 相似文献
74.
Gustav Wendelberger 《Plant Systematics and Evolution》1943,92(3):124-144
Ohne Zusammenfassung 相似文献
75.
Zusammenfassung Es wurde eine eindeutige sichere Methode ausgearbeitet, um das Kokain in der Pflanze mikrochemisch zu fassen und zu verfolgen. Der sicherste Nachweis läßt sich erbringen durch Mikrosublimation des Kokains und Nachweis im Sublimate mit Au Cl3 + K Br oder Extraktion mit CHCl3 + NH3 und Reaktion mit Au Cl3 + K Br. Daneben lassen sich noch verwenden die Benzoesäuresublimation und die direkte Reaktion im Schnitt, die jedoch nur bei Anwesenheit von größeren Mengen Kokain gute Resultate geben. 相似文献
76.
Gustav Peinel 《Chemistry and physics of lipids》1975,14(3):268-273
A modified CNDO-APSG procedure to obtain the charge distributions of the headgroups of phosphatidylserine, -ethanolamine and -choline as monomers and as constituents of hexagonal lattice is presented. The calculated charge distributions are typically zwitter-ionic. There is only a small difference between the charges for the monomer and as constituent of the lattice, from which it may be assumed that the main characteristics of the headgroups are the same in both cases. 相似文献
77.
Ohne Zusammenfassung 相似文献
78.
79.
A smooth microsomal fraction (smooth II microsomes) was earlier isolated and characterized in a number of investigations. Using a three-layer discontinuous sucrose gradient containing Mg2+ this fraction was divided into two subfractions (IIa and IIb) by a single centrifugation. The smooth IIa fraction proved to be a purified smooth microsomal fraction of specific composition. It contains high amounts of cytochromes and P-450, low activities of other electron transport enzymes and glucose-6-phosphatase, and no UDP-glucuronic acid transferase. No membrane or enzyme synthesis is induced in this subfraction by treatment with phenobarbital or methylcholanthrene. It appears that the membranes of smooth IIa microsomes derive from the smooth endoplasmic reticulum and are devoted to specific functions. 相似文献
80.
The transport and distribution of apo- and holocytochrome was investigated with the aid of specific antibodies. The holoenzyme was found to be localized mainly in the rough and smooth endoplasmic reticulum and in the Golgi system but some precipitation could also be obtained in the outer mitochondrial membranes and in the peroxisomes. The apoenzyme, however, could only be detected in the endoplasmic reticulum-Golgi system, which also was shown to be the sole site for incorporation of the prosthetic heme moiety. Time-course studies revealed that the labeled enzyme appeared both as apoenzyme and as holoenzyme in the rough endoplasmic reticulum 10 min after in vivo injection of radioactive leucine and that further transport to the smooth endoplasmic reticulum occurred within 10 min. The subsequent transport to other organelles, however, required a somewhat longer time and peak radioactivity in outer mitochondrial membranes was not attained until after 40 min. 相似文献